Molecular Food Science Laboratory, College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou 310018, China.
Acon Biotech (Hangzhou) Co., Ltd., Hangzhou 310030, China.
Biosensors (Basel). 2024 Oct 16;14(10):505. doi: 10.3390/bios14100505.
Triton X-100 (TX-100) is a commonly used surfactant in the manufacture of biosensors. The factors limiting the use of TX-100 in biosensors are environmental concerns. In this study, the binary system of sodium dodecyl benzene sulfonate (SDBS) and fatty alcohol-polyoxyethlene ether (AEO) was investigated from the physicochemical principle of surfactant interaction and its application in biosensors. The results demonstrated that a mixture of SDBS and AEO at an appropriate molar ratio had a comparable activity to TX-100 in terms of surface activity, micelle formation, dynamic adsorption, foaming, emulsifying, and cell permeability. Theory and experimentation support the idea that SDBS-AEO might take the place of TX-100 in the manufacturing of biosensors. This study contributes to the development of alternatives to TX-100 and provides a new perspective for an in-depth study of the interaction mechanism of additives in biosensor design.
Triton X-100（TX-100）是生物传感器制造中常用的表面活性剂。限制 TX-100 在生物传感器中使用的因素是环境问题。在这项研究中，从表面活性剂相互作用的物理化学原理及其在生物传感器中的应用出发，研究了十二烷基苯磺酸钠（SDBS）和脂肪醇聚氧乙烯醚（AEO）的二元体系。结果表明，在适当的摩尔比下，SDBS 和 AEO 的混合物在表面活性、胶束形成、动态吸附、发泡、乳化和细胞通透性方面与 TX-100 具有相当的活性。理论和实验都支持 SDBS-AEO 可能取代 TX-100 用于生物传感器制造的观点。本研究为替代 TX-100 提供了思路，为深入研究生物传感器设计中添加剂的相互作用机制提供了新的视角。
