Zhao Wenyuan, Du Ewei, Luo Rongchao, Chen Yaping, Sun Zhongxiang, Gui Furong
State Key Laboratory for Conservation and Utilization of Bioresources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, China.
Graduate School, Yunnan Agricultural University, Kunming, 650201, China.
BMC Plant Biol. 2024 Dec 23;24(1):1236. doi: 10.1186/s12870-024-05952-2.
Tuta absoluta (Lepidoptera: Gelechiidae) is one of the most significant invasive and destructive pests worldwide, causing serious economic losses to the tomato industry. Rhizosphere microorganism, such as arbuscular mycorrhizal fungi (AMF) and Pseudomonas bacteria, can interact with plants individually or collectively to improve plant growth and resistance to pests and disease. However, the effects of AMF, Pseudomonas, and their interactions on plant responses to insect herbivores remain unclear. A pot experiment was conducted to investigate the effects of single/dual inoculation with AMF (Funneliformis mosseae, M) and Pseudomonas putida (P) on the growth and defense of tomato variety Dafen (Solanum lycopersicum L.) in response to infestation by T. absoluta, as well as the growth, development, and enzyme activity of insect. The results showed that M, P, and MP promoted tomato growth by increasing nutrient concentrations, with the growth-promoting effect of dual-inoculation significantly surpassing that of single inoculation. M, P, and MP still improved tomato growth in T. absoluta infestation, with biomass increases of 57.34%, 54.46%, and 255.49%. M, P, and MP significantly increased the defense ability of tomato, with jasmonic acid concentrations increasing by 42.15%, 60.87% and 90.02%, and phenylalanine ammonia-lyase activity increasing by 47.40%, 47.68%, and 59.97%. The inoculation treatments inhibited the growth and development of T. absoluta, reduced its feeding, prolonged its growth and development, decreased egg weight, and increased the activity of protective and detoxifying enzymes. Overall, our results indicated that AMF and bacteria can stimulate each other, positively influence tomato growth and enhance resistance to T. absoluta. These findings indicate the feasibility of AMF and bacteria in combinations as potential biocontrol agents for the management of T. absoluta.
番茄潜叶蛾（鳞翅目：麦蛾科）是全球最具危害性的入侵害虫之一，给番茄产业造成了严重的经济损失。根际微生物，如丛枝菌根真菌（AMF）和假单胞菌属细菌，能够单独或共同与植物相互作用，促进植物生长并增强植物对病虫害的抗性。然而，AMF、假单胞菌及其相互作用对植物响应昆虫食草作用的影响尚不清楚。本研究通过盆栽试验，探究了单独/双重接种AMF（摩西斗管囊霉，M）和恶臭假单胞菌（P）对番茄品种大粉（Solanum lycopersicum L.）生长和防御的影响，以及对番茄潜叶蛾生长、发育和酶活性的影响，该番茄品种受番茄潜叶蛾侵害。结果表明，M、P和MP通过增加养分浓度促进番茄生长，双重接种的促生长效果显著优于单一接种。在番茄潜叶蛾侵害的情况下，M、P和MP仍能促进番茄生长，生物量分别增加57.34%、54.46%和255.49%。M、P和MP显著提高了番茄的防御能力，茉莉酸浓度分别增加42.15%、60.87%和90.02%，苯丙氨酸解氨酶活性分别增加47.40%、47.68%和59.97%。接种处理抑制了番茄潜叶蛾的生长发育，减少了其取食量，延长了其生长发育时间，降低了卵重，并增加了保护酶和解毒酶的活性。总体而言，我们的结果表明，AMF和细菌可以相互刺激，对番茄生长产生积极影响，并增强对番茄潜叶蛾的抗性。这些发现表明，AMF和细菌组合作为番茄潜叶蛾潜在生物防治剂具有可行性。
