Gelen Volkan, Başeğmez Mehmet, Dursun İnan, Çinar Irfan, Kara Adem
Department of Physiology, Faculty of Veterinary Medicine Kafkas University Kars Türkiye.
Acıpayam Vocational High School, Department of Veterinary, Laboratory and Veterinary Health Program Pamukkale University Denizli Türkiye.
Food Sci Nutr. 2025 Apr 22;13(4):e70194. doi: 10.1002/fsn3.70194. eCollection 2025 Apr.
Doxorubicin (DOX) is the most widely used chemotherapeutic agent to treat various tumors. DOX treatment can damage many organs, including the brain, by causing oxidative stress. Several antioxidant substances can lessen the effects of DOX or make antioxidant defense systems work faster. Propolis (PROP) is a powerful agent with various healing effects, including antioxidant, antiproliferative, and anti-inflammatory. The point of this study is to look at the histopathological changes, apoptosis, and antioxidant effects of DOX on brain damage in rats. To find out what kinds of phytochemicals were in PROP from the Karlıova region of Bingöl province, ultra-high-performance liquid chromatography (UHPLC-Orbitrap-HRMS) was used. Then, we made an ethanol extract of it. A total of 28 healthy male Wistar albino rats, each 12 weeks old and weighing between 220 and 250 g, were included in the study. Rats were divided into four groups: control, PROP, DOX, and PROP+DOX. We applied the relevant treatments to the determined groups. Following the application, we decapitated the rats under the appropriate conditions and collected blood and brain tissue samples. We examined oxidative stress parameters in blood samples and used brain tissue samples for histopathological, biochemical, and molecular analyses. We determined DOX levels in the brain tissue samples using UHPLC-Orbitrap-HRMS. The findings obtained showed that the PROP extract improved DOX-induced brain tissue damage. In addition, PROP extract attenuated DOX-induced brain tissue inflammation, ER stress, apoptosis, and oxidative stress.
阿霉素（DOX）是治疗各种肿瘤最广泛使用的化疗药物。DOX治疗可通过引起氧化应激损害包括大脑在内的许多器官。几种抗氧化物质可以减轻DOX的影响或使抗氧化防御系统更快发挥作用。蜂胶（PROP）是一种具有多种治疗作用的强效物质，包括抗氧化、抗增殖和抗炎作用。本研究的目的是观察DOX对大鼠脑损伤的组织病理学变化、细胞凋亡和抗氧化作用。为了确定宾格尔省卡尔利奥瓦地区PROP中的植物化学物质种类，使用了超高效液相色谱法（UHPLC-Orbitrap-HRMS）。然后，我们制备了其乙醇提取物。本研究共纳入28只健康雄性Wistar白化大鼠，每只大鼠12周龄，体重在220至250克之间。大鼠分为四组：对照组、PROP组、DOX组和PROP+DOX组。我们对确定的组进行了相关治疗。治疗后，我们在适当条件下将大鼠断头，并采集血液和脑组织样本。我们检测了血液样本中的氧化应激参数，并将脑组织样本用于组织病理学、生化和分子分析。我们使用UHPLC-Orbitrap-HRMS测定脑组织样本中的DOX水平。所得结果表明，PROP提取物改善了DOX诱导的脑组织损伤。此外，PROP提取物减轻了DOX诱导的脑组织炎症、内质网应激、细胞凋亡和氧化应激。
