加蓬东南部半农村地区社区层面泌尿生殖系统血吸虫病的分子检测

Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon.

作者信息

Kouna Lady Charlène, Oyegue-Liabagui Sandrine Lydie, Atiga Chenis Nick, Mbani Mpega Ntigui Chérone Nancy, Imboumy-Limoukou Roméo Karl, Biteghe Bi Essone Jean Claude, Ontoua Steede Seinnat, Moukodoum Diamella Nancy, Okouga Alain Prince, Lekana-Douki Jean Bernard

机构信息

Unité d'Evolution, Epidémiologie et Résistances Parasitaires (UNEEREP), Centre Interdisciplinaire de Recherche Médicales de Franceville, Franceville BP 769, Gabon.

Ecole Doctorale Régionale d'Afrique Centrale en Infectiologie Tropicale, Franceville BP 876, Gabon.

出版信息

Diagnostics (Basel). 2025 Apr 22;15(9):1052. doi: 10.3390/diagnostics15091052.

DOI:10.3390/diagnostics15091052

PMID:40361870

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12071237/

Abstract

BACKGROUND

Urogenital schistosomiasis is endemic in Gabon. Our study aimed to detect the prevalence of urinary schistosomiasis and to evaluate the diagnostic performance of the qPCR technique compared to microscopy for the detection of at the community level in a semi-rural area.

METHOD

A cross-sectional survey was carried out. Urine samples were examined using Urine TICK test strips, a filtration technique, and qPCR. was detected by targeting the Dra1 gene.

RESULTS

The prevalence of urogenital schistosomiasis was determined and the performance of real-time PCR and urine strips was compared with that of urinary filtration. A total of 281 participants were enrolled in the study. The prevalence of urogenital schistosomiasis was increased slightly with the molecular technique (40.9%) compared to microscopy (36.7%), and the hematuria rate with Urine STICK was 33.5%. SAC (5-14 years old), Pre-SAC (>5 years old), and adolescents (15-17 years old) were the most affected group according to, respectively, whatever method was used. qPCR showed good agreement with microscopy, as well as excellent sensitivity (99.03%) and specificity (93.3). There was a good correlation between the number of eggs per 10 mL and the cycle threshold range.

CONCLUSION

These results show the importance of using a combination of diagnostic tools in the surveillance of schistosomiasis, particularly in preschool children, adolescents, women of childbearing age, and chronic and asymptomatic cases.

摘要

背景

泌尿生殖系统血吸虫病在加蓬呈地方性流行。我们的研究旨在检测泌尿血吸虫病的患病率，并在半农村地区的社区层面评估定量聚合酶链反应（qPCR）技术与显微镜检查相比在检测方面的诊断性能。

方法

开展了一项横断面调查。使用尿液TICK试纸条、一种过滤技术和qPCR对尿液样本进行检测。通过靶向Dra1基因来检测。

结果

确定了泌尿生殖系统血吸虫病的患病率，并将实时PCR和尿试纸条的性能与尿液过滤的性能进行了比较。共有281名参与者纳入研究。与显微镜检查（36.7%）相比，分子技术检测到的泌尿生殖系统血吸虫病患病率略有上升（40.9%），尿试纸条检测到的血尿率为33.5%。无论采用何种方法，5 - 14岁的学龄儿童、学龄前儿童（>5岁）和青少年（15 - 17岁）是受影响最严重的群体。qPCR与显微镜检查显示出良好的一致性，以及出色的敏感性（99.03%）和特异性（93.3）。每10毫升尿液中的虫卵数量与循环阈值范围之间存在良好的相关性。