Zhang Jun, Yang Fei, Zhang Aihong, Guo Qinggang, Sun Xiangrui, Zhang Shangqing, Di Dianping
Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences, Ministry of Agriculture and Rural Affairs, China/IPM Innovation Center of Hebei Province, International Science and Technology Joint Research Center on IPM of Hebei Province, No. 437 Dongguan Street, Baoding 071000, China.
Tangshan Academy of Agricultural Sciences, Tangshan 063000, China.
Microorganisms. 2025 May 14;13(5):1128. doi: 10.3390/microorganisms13051128.
Tomato is a widely cultivated vegetable crop worldwide. It is susceptible to various phytopathogens, including fungi, bacteria, viruses, and nematodes. In 2024, an unknown leaf spot disease outbreak, characterized by distinct brown necrotic lesions on leaves, was observed in tomato plants in Yunnan Province, China. Through rigorous pathogen isolation and the fulfillment of Koch's postulates, it was proved that the fungal isolate could infect tomato leaves and cause typical symptoms. The pathogen isolated from tomato leaves was identified as based on its morphology and using a multilocus sequence analysis method with the internal transcribed spacer gene (ITS1), beta-tubulin gene (TUB2), and translation elongation factor 1-alpha gene (TEF1-α). This represents the first documented case of infecting tomatoes in the world. Given the damage caused by to tomato plants, we explored biocontrol methods. Through a dual-culture assay on PDA plates, B31 demonstrated significant biocontrol potential, exhibiting strong antagonistic activity toward . In addition, we developed a polyethylene glycol (PEG)-mediated transformation system that successfully introduced pYF11-GFP into the protoplasts of . This achievement provides a foundation for future genetic manipulation studies of .
番茄是一种在全球广泛种植的蔬菜作物。它易受多种植物病原体的侵害，包括真菌、细菌、病毒和线虫。2024年，在中国云南省的番茄植株上观察到一种不明叶斑病爆发，其特征是叶片上出现明显的褐色坏死病斑。通过严格的病原体分离和科赫法则的验证，证明了该真菌分离物能够感染番茄叶片并引起典型症状。从番茄叶片中分离出的病原体，根据其形态，并使用基于内转录间隔区基因（ITS1）、β-微管蛋白基因（TUB2）和翻译延伸因子1-α基因（TEF1-α）的多位点序列分析方法进行鉴定。这是世界上首次有文献记载该病原体感染番茄的案例。鉴于该病原体对番茄植株造成的损害，我们探索了生物防治方法。通过在PDA平板上的共培养试验，B31表现出显著的生物防治潜力，对该病原体表现出强烈的拮抗活性。此外，我们开发了一种聚乙二醇（PEG）介导的转化系统，成功地将pYF11-GFP导入该病原体的原生质体中。这一成果为该病原体未来的遗传操作研究奠定了基础。
