Li Xingfan, Yu Huimei, Guo Yang, Liu Jia, Yu Wei, Guo Xiaorui
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China; Key Laboratory of Soybean Molecular Design Breeding, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 150081, Harbin, China.
Plant Physiol Biochem. 2025 Jun 17;227:110170. doi: 10.1016/j.plaphy.2025.110170.
Light quality is a critical regulator of plant morphology and physiological metabolism, with spectral optimization emerging as a key strategy for enhancing bioactive compound production in medicinal plants. Catharanthus roseus, valued for its alkaloid content, exhibits significant metabolic plasticity in response to light conditions. This study examined the effects of two red-to-blue light ratios [2:1 (R2B1) and 7:1 (R7B1)] on morphophysiological responses, metabolite profiles, and alkaloid accumulation in C. roseus, using white light as a control. Both R2B1 and R7B1 altered plant morphology and enhanced alkaloid synthesis. R2B1 notably increased antioxidant enzyme activity, while R7B1 boosted non-enzymatic antioxidants and phenolic compounds. The Integrative Biomarker Response version 2 analysis identified R7B1 as the optimal treatment for promoting the accumulation of bioactive compounds. Untargeted metabolomics revealed that R7B1 enhanced sugar levels and induced tissue-specific changes in organic acid metabolism. Correlation analysis suggested that red-to-blue light promotes alkaloid biosynthesis by modulating antioxidant systems and key metabolic pathways. These findings highlight the potential of red-to-blue light optimization to enhance alkaloid production in C. roseus, providing valuable insights into the regulation of secondary metabolism and practical strategies for improving bioactive compound yields in medicinal plants.
光质是植物形态和生理代谢的关键调节因子，光谱优化已成为提高药用植物生物活性化合物产量的关键策略。长春花因其生物碱含量而受到重视，对光照条件表现出显著的代谢可塑性。本研究以白光为对照，考察了两种红蓝光比例[2:1（R2B1）和7:1（R7B1）]对长春花形态生理反应、代谢物谱和生物碱积累的影响。R2B1和R7B1均改变了植物形态并增强了生物碱合成。R2B1显著提高了抗氧化酶活性，而R7B1提高了非酶抗氧化剂和酚类化合物的含量。综合生物标志物反应版本2分析确定R7B1是促进生物活性化合物积累的最佳处理。非靶向代谢组学表明，R7B1提高了糖水平，并诱导了有机酸代谢的组织特异性变化。相关性分析表明，红蓝光通过调节抗氧化系统和关键代谢途径促进生物碱生物合成。这些发现突出了红蓝光优化在提高长春花生物碱产量方面的潜力，为次生代谢调控提供了有价值的见解，并为提高药用植物生物活性化合物产量提供了实用策略。
