Gao Feng, Zhu Jingyi, Xue Xin, Chen Hongqi, Nong Xiaojin, Yang Chunling, Shen Weimin, Gan Pengfei
Anyang Academy of Agriculture Sciences, Anyang 455000, China.
State Key Laboratory of Crop Stress Resistance and High-Efciency Production, College of Plant Protection, Northwest A&F University, Xianyang 712100, China.
Int J Mol Sci. 2025 Jun 10;26(12):5538. doi: 10.3390/ijms26125538.
Wheat ( L.) is the world's most indispensable staple crop and a vital source of food for human diet. Wheat stripe rust, caused by f. sp. (), constitutes a severe threat to wheat production and in severe cases, the crop fails completely. Anmai1350 (AM1350) is moderately resistant to leaf rust and powdery mildew, and highly susceptible to sheath blight and fusarium head blight. We found that the length and area of mycelium in AM1350 cells varied at different time points of infection. To investigate the molecular mechanism of AM1350 resistance to , we performed transcriptome sequencing (RNA-seq). In this study, we analyzed the transcriptomic changes of the seedling leaves of AM1350 at different stages of infection at 0 h post-infection (hpi), 6 hpi, 24 hpi, 48 hpi, 72 hpi, and 120 hpi through RNA-seq. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) was used to validate RNA-seq data. It was determined that there were differences in the differentially expressed genes (DEGs) of AM1350, and the upregulation and downregulation of the DEGs changed with the time of infection. At different time points, there were varying degrees of enrichment in the response pathways of AM1350, such as the "MAPK signaling pathway-plant", the "plant-pathogen interaction" pathway and other pathways. After infected AM1350, the reactive oxygen species (ROS) content gradually increases. The ROS is toxic to , promotes the synthesis of phytoalexins, and inhibits the spread of . As a result, AM1350 shows resistance to race CYR34. The main objective of this study is to provide a better understanding for resistance mechanisms of wheat in response to infections and to avoid production loss.
小麦（L.）是世界上最重要的主食作物，也是人类饮食中至关重要的食物来源。由小麦条锈菌（Puccinia striiformis f. sp. tritici）引起的小麦条锈病对小麦生产构成严重威胁，在严重情况下，作物会完全绝收。安麦1350（AM1350）对叶锈病和白粉病具有中等抗性，对纹枯病和赤霉病高度敏感。我们发现，在条锈菌感染的不同时间点，AM1350细胞中菌丝体的长度和面积有所不同。为了探究AM1350对条锈菌的抗性分子机制，我们进行了转录组测序（RNA-seq）。在本研究中，我们通过RNA-seq分析了AM1350幼苗叶片在条锈菌感染后0小时（hpi）、6 hpi、24 hpi、48 hpi、72 hpi和120 hpi不同阶段的转录组变化。采用实时定量聚合酶链反应（qRT-PCR）验证RNA-seq数据。结果表明，AM1350的差异表达基因（DEG）存在差异，且DEG的上调和下调随感染时间而变化。在不同时间点，AM1350的响应途径存在不同程度的富集，如“MAPK信号通路-植物”、“植物-病原体相互作用”途径等。条锈菌感染AM1350后，活性氧（ROS）含量逐渐增加。ROS对条锈菌有毒害作用，促进植保素的合成，并抑制条锈菌的扩散。因此，AM1350对条锈菌小种CYR34表现出抗性。本研究的主要目的是更好地理解小麦对条锈菌感染的抗性机制，避免产量损失。
