Gang D R, Weber D J
Brigham Young University, Provo, Utah, USA.
Biotechniques. 1995 Jul;19(1):92,94, 96-7.
We describe a method for isolating genomic DNA from teliospores of Tilletia caries (DC) Tul., T. controversa Kuhn and T. foetida (T. laevis) (Wallr.) Liro. for random-amplified polymorphic DNA (RAPD) analysis. DNA analysis of teliospores of covered smut or bunt has been difficult because of the thick wall and the high lipid content of the spores. This method overcomes these problems and yields sufficient quantities of DNA from the three species' teliospores for RAPDs. DNA quality appears to be good with very little degradation. RAPD amplifications of the extracted DNAs are reproducible and produce numerous large molecular weight bands from each individual. This procedure should permit the use of DNA analysis techniques to study species and races of Tilletia as well as fungi with similar spore structure.
我们描述了一种从小麦网腥黑粉菌（DC）Tul.、小麦矮腥黑粉菌Kuhn和小麦光腥黑粉菌（T. laevis）（Wallr.）Liro.的冬孢子中分离基因组DNA用于随机扩增多态性DNA（RAPD）分析的方法。由于厚垣孢子壁厚且脂质含量高，对腥黑粉病或腥黑穗病冬孢子进行DNA分析一直很困难。该方法克服了这些问题，从这三个物种的冬孢子中获得了足够数量的DNA用于RAPD分析。DNA质量似乎很好，几乎没有降解。提取的DNA的RAPD扩增具有可重复性，并且从每个个体中产生大量的大分子质量条带。该方法应能使DNA分析技术用于研究腥黑粉菌以及具有类似孢子结构的真菌的物种和生理小种。
