Harrison F W, Zambernard J, Cowden R R
Acta Histochem. 1975;54(2):295-306.
Fluorescent cytochemical techniques, chiefly end-group methods, have been employed in the characterization of cells in calid and algid normal and LUCKE tumor-bearing kidneys of the leopard frog, Rana pipiens. Cytoplasmic RNA and glycogen distribution reflect the known patterns of general metabolic activity of the respective cells in question. Protein sulfhydryl distribution is similar in normal distal tubules and tumors while both differ significantly from proximal tubules. Viral inclusions in nuclei of algid tumor cells contain demonstrable DNA, protein sulfhydryls and disulfides, arginine and lysine-rich basic proteins, also both C-terminal and side chain carboxyl groups. Significant amounts of DNA are demonstrable in the cytoplasm of algid tumors. Fluorescence cytochemical methods directed toward end-groups or specific classes of macromolecules offer a particularly favorable option, because of increased sensitivity and higher levels of contrast, for comparative cytological investigations of the LUCKE tumor and of similar systems.
荧光细胞化学技术，主要是端基法，已被用于表征豹蛙（Rana pipiens）温热和寒冷状态下正常以及患卢基氏瘤的肾脏中的细胞。细胞质RNA和糖原分布反映了相关各细胞已知的一般代谢活动模式。正常远端小管和肿瘤中的蛋白质巯基分布相似，而两者均与近端小管有显著差异。寒冷状态下肿瘤细胞的细胞核中的病毒包涵体含有可证实的DNA、蛋白质巯基和二硫键、富含精氨酸和赖氨酸的碱性蛋白质，以及C末端和侧链羧基。在寒冷状态下肿瘤的细胞质中可证实有大量DNA。由于灵敏度提高和对比度更高，针对端基或特定类大分子的荧光细胞化学方法为卢基氏瘤及类似系统的比较细胞学研究提供了一个特别有利的选择。
