低亲和力抗体在近期实验性血吸虫感染诊断中的应用
Low avidity antibodies in diagnosis of recent experimental schistosomal infection.
作者信息
Hassan M M, Farghaly A M, Abdel-Fattah M M, Seleem M A
机构信息
Department of Parasitology, Faculty of Medicine, Zagazig University, Egypt.
出版信息
J Egypt Soc Parasitol. 1994 Apr;24(1):193-8.
ELISA test was used to measure both of low- and high-avidity specific IgG against soluble S. mansoni egg antigen, to differentiate between S. mansoni experimentally infected mice with recent and chronic infection. Mice were infected with 100 S. mansoni cercariae and serum samples were obtained and tested 4, 6, 8, 10, 12, 14 and 16 weeks after infection. The results showed that there was a statistically significant difference between low- and high-avidity specific IgG in experimentally infected mice at 4, 6, 8, and 10 weeks after infection (recent infection). On the other hand, no difference was found in those with chronic infection (12, 14 and 16 weeks after infection). From these results it was concluded that the comparison of low- and high-avidity specific IgG levels was able to differentiate between recent and chronic experimentally infected mice with S. mansoni.
采用酶联免疫吸附测定（ELISA）试验检测针对可溶性曼氏血吸虫虫卵抗原的低亲和力和高亲和力特异性IgG，以区分近期感染和慢性感染曼氏血吸虫的实验感染小鼠。用100条曼氏血吸虫尾蚴感染小鼠，并在感染后4、6、8、10、12、14和16周采集血清样本进行检测。结果显示，在感染后4、6、8和10周（近期感染）的实验感染小鼠中，低亲和力和高亲和力特异性IgG之间存在统计学显著差异。另一方面，在慢性感染小鼠（感染后12、14和16周）中未发现差异。从这些结果得出结论，低亲和力和高亲和力特异性IgG水平的比较能够区分近期感染和慢性实验感染曼氏血吸虫的小鼠。
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