Baskar J F, Furnari B, Huang E S
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill.
J Infect Dis. 1993 Jun;167(6):1288-95. doi: 10.1093/infdis/167.6.1288.
To study the potential consequences of sperm-mediated sexual cytomegalovirus (CMV) transmission, an in vitro model of microinjection of murine CMV (MCMV) DNA into uninfected fertilized murine ova was used. After microinjected ova were cultured to blastocysts and transferred to pseudopregnant mice, the effect of the DNA on implantation and development was analyzed. At various times, the sites of implantation in the endometrium were examined. Reductions in litter size, fetal growth retardation, resorption of embryos, and fetal maldevelopment, which often involved the central nervous system, were observed. The presence of MCMV DNA sequences in DNA derived from fetuses was detected by the polymerase chain reaction followed by oligonucleotide hybridization. By in situ DNA-DNA cytohybridization and indirect immunofluorescence assays the viral sequences and antigens were localized primarily to the brain, salivary gland, and skin of maldeveloped fetuses. These results establish the potential consequences of sperm-mediated CMV transmission and induction of fetal anomalies.
为了研究精子介导的性传播巨细胞病毒（CMV）的潜在后果，采用了将鼠巨细胞病毒（MCMV）DNA显微注射到未感染的受精鼠卵中的体外模型。显微注射后的卵培养至囊胚并转移到假孕小鼠体内后，分析DNA对着床和发育的影响。在不同时间检查子宫内膜中的着床部位。观察到窝仔数减少、胎儿生长迟缓、胚胎吸收以及胎儿发育不良，后者常累及中枢神经系统。通过聚合酶链反应继之以寡核苷酸杂交检测胎儿来源的DNA中MCMV DNA序列的存在。通过原位DNA-DNA细胞杂交和间接免疫荧光测定，病毒序列和抗原主要定位于发育不良胎儿的脑、唾液腺和皮肤。这些结果证实了精子介导的CMV传播及胎儿异常诱导的潜在后果。
