Alberico P, Porta R, Pescador R, Ferro L
CRINOS Biological Research Laboratories, Villa Guardia (CO), Italy.
Thromb Res. 1995 Nov 15;80(4):281-9. doi: 10.1016/0049-3848(95)00178-t.
Defibrotide both "in vitro" and "ex vivo" decreases PMN adhesion to nylon columns. This activity is blunted by 8-phenyltheophylline, a known blocker of adenosine A1/A2 receptors, suggesting that Defibrotide acts on that kind of receptors. Indomethacin, a blocker of PGI2 synthesis, did not cancel DFT's activity, suggesting that PGI2 is not involved in DFT's activity, at least under our experimental conditions. Non-PMN leukocytes behaved differently "ex vivo" than PMNs. DFT's activity was not blunted by 8-phenyltheophylline, suggesting that DFT acts on this kind of cell through a different mechanism. The "in vitro" experiment did not confirm "ex vivo" studies suggesting that in the "in vitro" experiment "something" is missing that is present in "ex vivo" experiments.
去纤苷在“体外”和“离体”条件下均能降低中性粒细胞与尼龙柱的黏附。8-苯基茶碱是一种已知的腺苷A1/A2受体阻滞剂，该活性会被其减弱，这表明去纤苷作用于这类受体。吲哚美辛是前列环素I2（PGI2）合成的阻滞剂，它并未消除去纤苷的活性，这表明至少在我们的实验条件下，PGI2不参与去纤苷的活性。非中性粒细胞在“离体”条件下的行为与中性粒细胞不同。去纤苷的活性未被8-苯基茶碱减弱，这表明去纤苷通过不同机制作用于这类细胞。“体外”实验并未证实“离体”研究结果，这表明在“体外”实验中缺少“离体”实验中存在的“某些因素”。
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