Noyes H, Chance M, Ponce C, Ponce E, Maingon R
Liverpool School of Tropical Medicine, UK.
Exp Parasitol. 1997 Mar;85(3):264-73. doi: 10.1006/expr.1996.4133.
In the Mediterranean region Leishmania infantum causes both visceral and cutaneous leishmaniasis. These two pathologies tend to be caused by distinct parasite zymodemes. We have studied 33 isolates of Leishmania, 2 from sandflies, 5 from visceral cases, and 26 from cutaneous cases in Honduras, to determine if there is a correlation between pathology and parasite type in the New World similar to that in the Mediterranean region. Nine of the 26 cutaneous cases were caused by L. mexicana parasites, which have not been previously reported from Honduras; the remaining 17 cutaneous cases were due to L. chagasi. Only minor differences were found between the Honduran L. chagasi parasites by random amplified polymorphic DNA, differential display, pulsed-field gel electrophoresis, and schizodemes. This suggests that in Honduras the parasite type may not be the only factor determining the clinical outcome of L. chagasi infections.
在 Mediterranean 地区，婴儿利什曼原虫可引发内脏利什曼病和皮肤利什曼病。这两种病症往往由不同的寄生虫酶谱型引起。我们研究了来自洪都拉斯的 33 株利什曼原虫，其中 2 株来自白蛉，5 株来自内脏病例，26 株来自皮肤病例，以确定在新世界中病症与寄生虫类型之间是否存在类似于 Mediterranean 地区的相关性。26 例皮肤病例中有 9 例由墨西哥利什曼原虫寄生虫引起，此前洪都拉斯尚未有过相关报道；其余 17 例皮肤病例由恰加斯利什曼原虫所致。通过随机扩增多态性 DNA、差异显示、脉冲场凝胶电泳和裂殖体酶谱分析，发现洪都拉斯的恰加斯利什曼原虫寄生虫之间仅有微小差异。这表明在洪都拉斯，寄生虫类型可能并非决定恰加斯利什曼原虫感染临床结果的唯一因素。
