Santoro B, Liu D T, Yao H, Bartsch D, Kandel E R, Siegelbaum S A, Tibbs G R
Center for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA.
Cell. 1998 May 29;93(5):717-29. doi: 10.1016/s0092-8674(00)81434-8.
The generation of pacemaker activity in heart and brain is mediated by hyperpolarization-activated cation channels that are directly regulated by cyclic nucleotides. We previously cloned a novel member of the voltage-gated K channel family from mouse brain (mBCNG-1) that contained a carboxy-terminal cyclic nucleotide-binding domain (Santoro et al., 1997) and hence proposed it to be a candidate gene for pacemaker channels. Heterologous expression of mBCNG-1 demonstrates that it does indeed code for a channel with properties indistinguishable from pacemaker channels in brain and similar to those in heart. Three additional mouse genes and two human genes closely related to mBCNG-1 display unique patterns of mRNA expression in different tissues, including brain and heart, demonstrating that these channels constitute a widely expressed gene family.
心脏和大脑中起搏器活动的产生是由超极化激活的阳离子通道介导的，这些通道直接受环核苷酸调节。我们之前从小鼠大脑中克隆了电压门控钾通道家族的一个新成员（mBCNG-1），它含有一个羧基末端环核苷酸结合结构域（Santoro等人，1997年），因此提出它是起搏器通道的候选基因。mBCNG-1的异源表达表明，它确实编码一种通道，其特性与大脑中的起搏器通道无法区分，且与心脏中的起搏器通道相似。另外三个小鼠基因和两个与mBCNG-1密切相关的人类基因在不同组织（包括大脑和心脏）中表现出独特的mRNA表达模式，表明这些通道构成了一个广泛表达的基因家族。
