人体淋巴细胞和成纤维细胞培养物中纺锤体装置的染色

Hum Genet. 1976 Oct 28;34(2):195-7. doi: 10.1007/BF00278888.

The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol--acetic acid and treated for about 15 min with 0.1 X SSC at 92 degrees C and for 18 h with 2 X SSC at 60 degrees C.

如果用人的有丝分裂细胞用甲醇 - 乙酸固定，然后在92℃下用0.1X SSC处理约15分钟，在60℃下用2X SSC处理18小时，那么人有丝分裂的纺锤体装置可以用吉姆萨染色法进行优先染色。