C反应蛋白增强虹鳟鱼头肾细胞的吞噬和趋化活性。

Enhancement of phagocytic and chemokinetic activities of rainbow trout head kidney cells by C-reactive protein.

作者信息

Kodama H, Arimitsu H, Mukamoto M, Sugimoto C

机构信息

Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Sakai, Japan.

出版信息

Am J Vet Res. 1999 Feb;60(2):240-4.

PMID:10048559

Abstract

OBJECTIVE

To investigate immunostimulating activity of purified rainbow trout (Oncorhynchus mykiss) C-reactive protein (CRP) on trout phagocytic cells.

ANIMALS

20 rainbow trout and 2 rabbits.

PROCEDURE

The effect of CRP on phagocytic activity of head kidney (HK) cells was examined by use of a phagocytosis assay with plastic particles. The enhancing effect of CRP on migration activity of HK cells was examined by use of the blind well assay.

RESULTS

Glass-adherent cells from clinically normal trout had increased dose-dependent phagocytic activity against plastic particles when cells were incubated in the presence of CRP. Pretreatment of particles with CRP also enhanced phagocytic activity of the cells, indicating an opsonic effect of CRP. Rabbit anti-trout CRP serum suppressed the enhancing activity of CRP. The HK cells had significant dose-dependent chemokinetic activity against CRP that was not inhibited by anti-CRP serum, indicating that a CRP-antibody complex also could be chemokinetic.

CONCLUSIONS

Rainbow trout CRP has immunostimulating activity for HK cells, resulting in enhanced phagocytic and chemokinetic activities.

摘要

目的

研究纯化的虹鳟（Oncorhynchus mykiss）C反应蛋白（CRP）对虹鳟吞噬细胞的免疫刺激活性。

动物

20条虹鳟和2只兔子。

实验步骤

通过使用塑料颗粒吞噬试验检测CRP对头肾（HK）细胞吞噬活性的影响。通过使用盲孔试验检测CRP对HK细胞迁移活性的增强作用。

结果

当在CRP存在的情况下孵育细胞时，来自临床正常虹鳟的玻璃贴壁细胞对塑料颗粒的吞噬活性呈剂量依赖性增加。用CRP预处理颗粒也增强了细胞的吞噬活性，表明CRP具有调理作用。兔抗虹鳟CRP血清抑制了CRP的增强活性。HK细胞对CRP具有显著的剂量依赖性化学趋动活性，且不受抗CRP血清的抑制，表明CRP-抗体复合物也可能具有化学趋动作用。