Isolation of Prothoteca zopfii from a case of bovine mastitis in Brazil.

The isolation of Prothoteca zopfii, an algae lacking chlorophyll, from bovine mastitic milk, is described herein. The isolation was performed on 8% sheep blood agar, following incubation at 37 circC for 48 h. Based on biochemical tests, susceptibility to clotrimazole, and light and electron microscopic observation of cellular morphology the algae was identified as P. zopfii. The affected animal did not improve following treatment and had to be eliminated.