使用凝集素探针分析原发性干燥综合征患者的泪液。原发性干燥综合征患者、其他免疫炎性结缔组织病患者及对照的比较研究。
Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes. A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls.
作者信息
Bjerrum K B
机构信息
Department of Ophthalmology, Rigshospitalet and Eye Pathology Institute, University of Copenhagen, Denmark.
出版信息
Acta Ophthalmol Scand. 1999 Feb;77(1):1-8. doi: 10.1034/j.1600-0420.1999.770101.x.
PURPOSE
To study and compare the glycoprotein composition of tear fluid from patients with primary Sjögren's syndrome, patients with other connective tissue diseases and control individuals.
METHODS
SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins.
RESULTS
The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjögren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range.
CONCLUSIONS
A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjögren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test.
目的
研究并比较原发性干燥综合征患者、其他结缔组织病患者及对照个体泪液中的糖蛋白组成。
方法
采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳，随后进行考马斯亮蓝染色或蛋白质印迹法，并与十种不同的凝集素进行叠加。
结果
与对照组及其他结缔组织病患者相比，原发性干燥综合征患者中发现的许多糖蛋白条带的频率显著降低。这种降低在分子量为14 - 68 kDa的范围内最为明显。
结论
在原发性干燥综合征患者中发现泪液糖蛋白的产生率降低和/或糖基化模式改变。然而，所发现的变化特异性不足，无法作为诊断测试。
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