Oishi K, Yokoi M, Maekawa S, Sodeyama C, Shiraishi T, Kondo R, Kuriyama T, Machida K
Department of Hygiene and Public Health, School of Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.
Acta Physiol Scand. 1999 Jan;165(1):65-9. doi: 10.1046/j.1365-201x.1999.00482.x.
Immobilization stress induces formation of reactive oxygen species (ROS) and leads to the oxidative injury in various tissues. In this study, the effects of immobilization stress on peripheral blood cells distribution, plasma level of thiobarbituric acid reactive substances (TBARS), and activities of antioxidant enzymes in erythrocytes were investigated in male Fischer rats. A significant increase in plasma TBARS was observed during and after the stress. Dramatic increases of neutrophils and monocytes imply that ROS formation resulted from their activation. Furthermore, the antioxidant activities of catalase and superoxide dismutase (SOD) in erythrocytes were dramatically increased during and after the stress, while a large fall in erythrocyte number was observed. These findings suggest that the activation of immune cells can be a source of the immobilization-induced ROS production, and that antioxidant enzymes in erythrocytes play an important role in preventing the ROS-induced injuries.
制动应激会诱导活性氧（ROS）的形成，并导致各种组织发生氧化损伤。在本研究中，我们在雄性Fischer大鼠中研究了制动应激对外周血细胞分布、血浆中硫代巴比妥酸反应性物质（TBARS）水平以及红细胞中抗氧化酶活性的影响。在应激期间及应激后，观察到血浆TBARS显著增加。中性粒细胞和单核细胞的显著增加表明ROS的形成是由它们的激活所致。此外，在应激期间及应激后，红细胞中过氧化氢酶和超氧化物歧化酶（SOD）的抗氧化活性显著增加，同时观察到红细胞数量大幅下降。这些发现表明，免疫细胞的激活可能是制动诱导的ROS产生的一个来源，并且红细胞中的抗氧化酶在预防ROS诱导的损伤中起重要作用。
