毛细管亲和电泳作为研究生物分子相互作用的通用工具：一篇综述。

Capillary affinophoresis as a versatile tool for the study of biomolecular interactions: a mini-review.

作者信息

Shimura K, Kasai K

机构信息

Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.

出版信息

J Mol Recognit. 1998 Winter;11(1-6):134-40. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<134::AID-JMR409>3.0.CO;2-G.

DOI:10.1002/(SICI)1099-1352(199812)11:1/6<134::AID-JMR409>3.0.CO;2-G

Abstract

Combination of capillary electrophoresis and bioaffinity interaction gave rise to powerful research tools for analyzing molecular recognition. They take advantages of the electrophoretic behavior of the complex formed between a target biomolecule and a specially designed mobile ligand molecule (affinophore or affinity probe), and enable detection of complex formation, determination of the equilibrium constants and stoichiometry, etc.

摘要

毛细管电泳与生物亲和相互作用相结合，产生了用于分析分子识别的强大研究工具。它们利用目标生物分子与专门设计的流动配体分子（亲和体或亲和探针）形成的复合物的电泳行为，能够检测复合物的形成、测定平衡常数和化学计量比等。

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