用于检测莠去津的电化学免疫传感器。
Electrochemical immunosensor for the detection of atrazine.
作者信息
López M A, Ortega F, Domínguez E, Katakis I
机构信息
Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
出版信息
J Mol Recognit. 1998 Winter;11(1-6):178-81. doi: 10.1002/(SICI)1099-1352(199812)11:1/6<178::AID-JMR417>3.0.CO;2-5.
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).
一种用于检测除草剂阿特拉津的安培免疫传感器已被研制出来。氧化还原聚合物PVPOs(bpy)2Cl与特异性抗体通过与聚乙二醇二缩水甘油醚交联共同固定在电极表面，形成导电水凝胶。在竞争分析中，在0.1 V（相对于Ag/AgCl）的H2O2存在下，通过抗原标记的辣根过氧化物酶的氧化还原中心与电极表面的“电连接”来检测传感膜表面抗体 - 抗原反应的发生。
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