Takahashi M, Taguchi H, Yamaguchi H, Osaki T, Sakazaki R, Kamiya S
Miyarisan Pharmaceutical Co., Ltd. Kaminakazato, Tokyo, Japan.
Kansenshogaku Zasshi. 1999 Jan;73(1):7-14. doi: 10.11150/kansenshogakuzasshi1970.73.7.
Antagonistic interaction between Clostridium butyricum strain MIYAIRI 588 and enterohemorrhagic Esherichia coli (EHEC) strain O157:H7 006 was examined using streptomycin-treated SPF mice and germ free mice. All SPF mice pretreated with streptomycin were colonized with EHEC O157:H7. On the other hand, only 20% of the SPF mice pretreated with streptomycin and C. butyricum were colonized with EHEC O157:H7. In addition, germ free mice died within 4-7 days after infection with EHEC O157:H7. In contrast, all gnotobiotic mice mono-associated with C. butyricum survived after the challenge with EHEC O157:H7. Both the number of EHEC and the amounts of shiga-like cytotoxin (SLT, type 1 and type 2) in fecal contents of gnotobiotic mice treated with C. butyricum were less than those of mice infected with only EHEC O157:H7. In conclusion, the probiotic bacterium, C. butyricum strain MIYAIRI 588, has a preventive effect against EHEC O157:H7 infection.
使用链霉素处理的无特定病原体（SPF）小鼠和无菌小鼠，研究了丁酸梭菌MIYAIRI 588菌株与肠出血性大肠杆菌（EHEC）O157:H7 006菌株之间的拮抗相互作用。所有用链霉素预处理的SPF小鼠都被EHEC O157:H7定植。另一方面，在用链霉素和丁酸梭菌预处理的SPF小鼠中，只有20%被EHEC O157:H7定植。此外，无菌小鼠在感染EHEC O157:H7后4 - 7天内死亡。相比之下，所有与丁酸梭菌单关联的悉生小鼠在接受EHEC O157:H7攻击后都存活了下来。用丁酸梭菌处理的悉生小鼠粪便内容物中EHEC的数量以及志贺样细胞毒素（SLT，1型和2型）的量均低于仅感染EHEC O157:H7的小鼠。总之，益生菌丁酸梭菌MIYAIRI 588菌株对EHEC O157:H7感染具有预防作用。
