使用机器人工作站实现类固醇放射免疫分析的自动化。
Automation of steroid radioimmunoassays using a robotics workstation.
作者信息
Antonian L, Iacolina M D, Proulx R R
机构信息
Merck Research Laboratories, Department of Drug Metabolism, Rahway, NJ 07065, USA.
出版信息
Clin Lab Sci. 1995 Mar-Apr;8(2):117-20.
OBJECTIVE
To automate the radioimmunoassay (RIA) of steroids in a diagnostic clinical laboratory.
SETTING
The Laboratory of Pediatric Endocrinology, Cornell Medical Center-New York Hospital.
PRACTICE DESCRIPTION
A tertiary-care center supporting the diagnosis and treatment of pediatric patients with endocrine disorders. PRODUCT COMPARISON: The Packard MultiPROBE Model 100 Automated Liquid Handling System (Packard Instrument Company, Meriden, CT 06450) was compared with the assays typically used to assist in the diagnosis of steroidogenic disorders in children: extraction of biologic fluids, celite partition chromatography, and RIA.
MAIN OUTCOME MEASUREMENT
The comparative efficiency, cost-effectiveness, specificity, and sensitivity of the two methods.
RESULT
The automated RIAs correlated (r = 0.963) with the established manual assays for steroids. The specificity and sensitivity of the assays were uniformly maintained after automation.
CONCLUSION
The automation of steroid RIAs has made the method a highly efficient and cost-effective one for diagnostic clinical laboratories.
目的
在诊断性临床实验室实现类固醇放射免疫分析（RIA）的自动化。
地点
康奈尔医学中心 - 纽约医院儿科内分泌实验室。
业务描述
一家支持儿科内分泌疾病诊断和治疗的三级护理中心。产品比较：将帕卡德MultiPROBE 100型自动液体处理系统（帕卡德仪器公司，美国康涅狄格州梅里登06450）与通常用于协助诊断儿童类固醇生成障碍的检测方法进行比较：生物体液提取、硅藻土分配色谱法和RIA。
主要观察指标
两种方法的比较效率、成本效益、特异性和敏感性。
结果
自动化RIA与既定的类固醇手动检测方法具有相关性（r = 0.963）。自动化后检测的特异性和敏感性均得以保持。
结论
类固醇RIA的自动化使该方法成为诊断性临床实验室一种高效且具成本效益的方法。
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