Effect of non-permissive temperature on protein synthesis of frog virus 3 infected cells.

In the present paper we describe the kinetics of virus specific protein synthesis in FV3-infected BHK cells incubated at the non-permissive temperature of 33 degrees C. Some quantitative differences were detected, in comparison with proteins synthesized at permissive temperature (26 degrees C). Thereafter we studied the fate of polypeptides pulse labelled at non-permissive temperature, by shifting the infected cells to permissive temperature. In such experimental conditions infectious virus is readily produced; however the profiles of electrophoretically separated polypeptides did not differ remarkably from those obtained from cells maintained at 33 degrees C.