Cattaneo A, Biocca S
Neurosceince Program, International School for Advanced Studies (SISSA), Trieste, Italy.
Trends Biotechnol. 1999 Mar;17(3):115-21. doi: 10.1016/s0167-7799(98)01268-2.
The intracellular expression of antibodies in mammalian cells is a strategy to inhibit the in vivo function of selected molecules but is limited by the unpredictable behaviour of antibodies when intracellularly expressed. Recent advances in the field of antibody expression in Escherichia coli show that the introduction of mutations can improve the properties of some antibody domains, but the general applicability of this approach to intracellular antibodies remains to be proved. As a complement to rational approaches, we describe selection schemes in which antibodies are selected on the basis of their performance in vivo as intracellular antibodies.
在哺乳动物细胞中进行抗体的细胞内表达是一种抑制特定分子体内功能的策略，但受细胞内表达抗体时不可预测行为的限制。大肠杆菌中抗体表达领域的最新进展表明，引入突变可改善某些抗体结构域的特性，但这种方法对细胞内抗体的普遍适用性仍有待证明。作为对合理方法的补充，我们描述了基于抗体作为细胞内抗体在体内的表现来选择抗体的方案。
