水稻（Oryza sativa L.）中铁超氧化物歧化酶cDNA的分子克隆与特性分析
Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.).
作者信息
Kaminaka H, Morita S, Tokumoto M, Yokoyama H, Masumura T, Tanaka K
机构信息
Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Japan.
出版信息
Biosci Biotechnol Biochem. 1999 Feb;63(2):302-8. doi: 10.1271/bbb.63.302.
We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.
我们从水稻（Oryza sativa L.）中分离出了一个编码铁超氧化物歧化酶（Fe-SOD）的cDNA。推导的氨基酸序列由一个含255个氨基酸的多肽组成，在氨基末端残基中包括一个推定的转运肽（40个氨基酸）。该序列与已知的植物Fe-SOD相似，但未归类于已知Fe-SOD组。对在大肠杆菌中表达的部分纯化重组蛋白进行的金属分析和超氧化物歧化酶测定表明，该cDNA编码一种含铁超氧化物歧化酶。然而，这种超氧化物歧化酶活性不受过氧化氢处理的抑制，而过氧化氢预期会抑制已知的Fe-SOD活性。在所有检测的营养组织中均检测到水稻Fe-SOD的mRNA，在愈伤组织中尤其丰富，并通过光诱导强烈增加。这些结果表明，该cDNA编码水稻Fe-SOD，它显然与已知的植物Fe-SOD不同。
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