Colonna S, Gaggero N, Richelmi C, Pasta P
Centro CNR and Istituto di Chimica Organica, Facoltà di Farmacia, Università degli Studi di Milano, via Venezian 21, I-20133 Milano,
Trends Biotechnol. 1999 Apr;17(4):163-8. doi: 10.1016/s0167-7799(98)01288-8.
Peroxidases are ubiquitous oxidoreductases that use hydrogen peroxide or alkyl peroxides as oxidants. Advances have recently been made in using them to prepare, under mild and controlled conditions, chiral organic molecules that are valuable for the chemoenzymatic synthesis of a wide range of useful compounds. Horseradish peroxidase can be converted into a peroxygenative enzyme by molecular engineering. Chloroperoxidase, the most versatile peroxidase, behaves like a 'true' monooxygenase in sulfoxidations with molecular oxygen and an external reductant, with substantial increases in enantioselectivity and enzyme stability.
过氧化物酶是普遍存在的氧化还原酶,它们使用过氧化氢或烷基过氧化物作为氧化剂。最近在利用它们在温和且可控的条件下制备对多种有用化合物的化学酶合成有价值的手性有机分子方面取得了进展。辣根过氧化物酶可通过分子工程转化为加氧酶。氯过氧化物酶是用途最广泛的过氧化物酶,在与分子氧和外部还原剂进行的硫氧化反应中表现得像一种“真正的”单加氧酶,对映选择性和酶稳定性大幅提高。