Osinowa O, Salamon S
Aust J Biol Sci. 1976 Oct;29(4):335-9. doi: 10.1071/bi9760335.
The fertility results of two experiments are presented. In experiment 1, the semen was frozen in tris-fructose-EDTA or BF3 diluents at 0-25 X 10(9)/ml sperm concentration and extended after thawing with either seminal plasma (SP) or the freezing medium (FM) containing no cryoprotective agent. In the second experiment the semen was glycerolated by two methods, frozen at 1-0 X 10(9)/ml sperm concentration, and extended wtih FM before insemination. Fertility after double insemination within one oestrus with semen frozen in tris-fructose-EDTA or BF3 diluents varied depending on the medium used for extension of thawed semen. The farrowing rates for semen frozen in the former diluent with FM and SP post-thawing media were 4/8 and 1/8 respectively, and for semen frozen BF3 diluent with FM and SP post-thawing extenders 1/8 and 5/8. The mean farrowing for the 32 animals inseminasted was 34-4%. Pregnancies for semen frozen in tris-fructose-EDTA and glycerolated at 30 or 5 degrees C were 5/12 and 4/12 respectively, and for single and double inseminations 6/12 and 3/12 respectively. Of 24 animals inseminated 37-5% farrowed.
本文展示了两项实验的繁殖结果。在实验1中,精液在三羟甲基氨基甲烷-果糖-乙二胺四乙酸(tris-fructose-EDTA)或三氟化硼(BF3)稀释液中以0 - 25×10⁹/ml的精子浓度冷冻,解冻后用精浆(SP)或不含冷冻保护剂的冷冻培养基(FM)进行稀释。在第二项实验中,精液通过两种方法进行甘油化处理,以1 - 0×10⁹/ml的精子浓度冷冻,并在授精前用FM进行稀释。在一个发情期内用在tris-fructose-EDTA或BF3稀释液中冷冻的精液进行两次授精后的繁殖力,取决于解冻精液所使用的培养基。用前一种稀释液冷冻且解冻后用FM和SP培养基的精液产仔率分别为4/8和1/8,而用BF3稀释液冷冻且解冻后用FM和SP稀释剂的精液产仔率分别为1/8和5/8。接受授精的32只动物的平均产仔率为34 - 4%。在tris-fructose-EDTA中冷冻并在30℃或5℃甘油化的精液的妊娠率分别为5/12和4/12,单次和两次授精的妊娠率分别为6/12和3/12。接受授精的24只动物中有37 - 5%产仔。