非洲恶性疟原虫分离株对双氢青蒿素的体外敏感性及对青蒿素耐药的危险因素

In vitro susceptibility of African Plasmodium falciparum isolates to dihydroartemisinin and the risk factors for resistance to qinghaosu.

作者信息

Le Bras J

机构信息

Centre National de Référence pour la Chimiosensibilité du Paludisme, Laboratoire de Parasitologie, Université Paris V, Hôpital Bichat-Claude Bernard, Paris.

出版信息

Med Trop (Mars). 1998;58(3 Suppl):18-21.

PMID:10212892

Abstract

In vitro susceptibility to dihydroartemisinin (DHART) and to artemether of 476 Plasmodium falciparum fresh clinical isolates obtained from non immune travellers returning from Africa to France in 1993-1996 were analysed to search for natural resistance and cross resistance of these compounds with available antimalarials. The mean median inhibitory concentration (IC50) values for artemether and DHART were 2.69 nM and 1.17 nM, respectively. Nineteen isolates presented with a decreased IC50 to artemether or DHART. Chloroquine-resistant isolates were more susceptible to artemether (2.15 versus 3.26 nM) and DHART (0.876 versus 1.51 nM) than chloroquine-susceptible ones. Artemether and DHART responses were correlated (r2 = 0.599). The isolates identified as resistant to DHART or artemether in this study had IC50's close to the cut-off and limited cross-resistant profiles.

摘要

分析了1993年至1996年从非洲返回法国的非免疫旅行者中获得的476株恶性疟原虫新鲜临床分离株对双氢青蒿素（DHART）和蒿甲醚的体外敏感性，以寻找这些化合物与现有抗疟药的天然抗性和交叉抗性。蒿甲醚和DHART的平均半数抑制浓度（IC50）值分别为2.69 nM和1.17 nM。19株分离株对蒿甲醚或DHART的IC50降低。耐氯喹分离株比氯喹敏感分离株对蒿甲醚（2.15对3.26 nM）和DHART（0.876对1.51 nM）更敏感。蒿甲醚和DHART的反应相关（r2 = 0.599）。在本研究中鉴定为对DHART或蒿甲醚耐药的分离株的IC50接近临界值且交叉耐药谱有限。