Aho K, Palosuo T, Lukka M, Kurki P, Isomäki H, Kautiainen H, von Essen R
National Public Health Institute, Helsinki, Finland.
Scand J Rheumatol. 1999;28(2):113-6. doi: 10.1080/030097499442586.
We evaluated the sensitivity and prognostic value of an enzyme-linked immunosorbent assay (ELISA) for the measurement of antifilaggrin antibodies (AFA), using filaggrin purified from human skin as an antigen. The AFA test was applied to a series of 306 patients with various recent-onset inflammatory joint diseases. The results were compared to those of the conventional immunofluorescence tests for antikeratin antibody (AKA) and antiperinuclear factor (APF) and of the rheumatoid factor (RF) tests from a previous study. There was a very good agreement between the results of the tests for APF and AFA (kappa-value 0.79 in patients with peripheral poly/oligoarthritis). The agreement between the tests for AKA and AFA was significant but less pronounced (kappa-value 0.50). The AFA test detected 10/22 of the RF-negative erosive cases, particularly those with a large number of erosive joints. Thus, the test for AFA supplements RF in the prediction of erosiveness.
我们使用从人皮肤中纯化的丝聚蛋白作为抗原，评估了用于检测抗丝聚蛋白抗体（AFA）的酶联免疫吸附测定（ELISA）的敏感性和预后价值。将AFA检测应用于306例近期发病的各种炎性关节疾病患者。将结果与先前研究中抗角蛋白抗体（AKA）和抗核周因子（APF）的传统免疫荧光检测结果以及类风湿因子（RF）检测结果进行比较。APF检测和AFA检测结果之间具有非常好的一致性（外周多/寡关节炎患者的kappa值为0.79）。AKA检测和AFA检测之间的一致性显著但不太明显（kappa值为0.50）。AFA检测在22例RF阴性侵蚀性病例中检测出10例，特别是那些有大量侵蚀性关节的病例。因此，AFA检测在侵蚀性预测方面补充了RF检测。
