Vacula R, Steiner J M, Krajcovic J, Ebringer L, Löffelhardt W
Institute of Molecular and Subcellular Biology, Comenius University, Bratislava, Slovak Republic.
DNA Res. 1999 Feb 26;6(1):45-9. doi: 10.1093/dnares/6.1.45.
The complete presequences of the nucleus-encoded precursors to two proteins, cytochrome c6 and the 30-kDa protein of the oxygen-evolving complex, that reside in the thylakoid lumen of the chloroplasts of Euglena gracilis are presented. Sorting of these proteins involves translocation across four membranes, the three-membraned chloroplast envelope and the thylakoid membrane. The tripartite presequences show the structure: signal sequence transit sequence signal sequence. Three hydrophobic domains become apparent: two of them correspond to signal sequences for translocation across the endoplasmic reticulum (ER) membrane and the thylakoid membrane, respectively, whereas the third constitutes the stop-transfer signal contained in the long stroma-targeting part of the tripartite presequence.
本文展示了纤细裸藻叶绿体类囊体腔中两种蛋白质（细胞色素c6和放氧复合体的30 kDa蛋白质）的细胞核编码前体的完整前导序列。这些蛋白质的分选涉及穿过四层膜，即三层膜的叶绿体被膜和类囊体膜。三方前导序列呈现出信号序列-转运序列-信号序列的结构。三个疏水结构域变得明显：其中两个分别对应于穿过内质网（ER）膜和类囊体膜的信号序列，而第三个则构成三方前导序列长的基质靶向部分中所含的停止转移信号。
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