一种与人类β2-微球蛋白结合的单链抗体噬菌体克隆。

An scFv phage clone that binds to human beta2-microglobulin.

作者信息

Jwang B, Yerushalmi N, Kreitman R J, Engberg J, Pastan I

机构信息

Laboratory of Molecular Biology, DBS, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Immunotechnology. 1999 Mar;4(3-4):231-6. doi: 10.1016/s1380-2933(98)00023-2.

DOI:10.1016/s1380-2933(98)00023-2

PMID:10231092

Abstract

Using phage display technology, a novel mouse scFv phage clone D1 was generated from mice immunized with single-chain HLA-A2, the human MHC class I molecules. D1 recognized a determinant in beta2-microglobulin. It reacted with beta2-microglobulin associated with the heavy chain of HLA-A2, recombinant beta2-microglobulin made in Escherichia coli or beta2-microglobulin prepared from human urine. The heavy chain variable region of D1 belongs to gene family VII and the light chain variable region belongs to the kappa chain gene family XI according to the Kabat database.

摘要

利用噬菌体展示技术，从用单链HLA-A2（人类MHC I类分子）免疫的小鼠中产生了一种新型小鼠单链抗体片段（scFv）噬菌体克隆D1。D1识别β2-微球蛋白中的一个决定簇。它能与与HLA-A2重链相关的β2-微球蛋白、在大肠杆菌中制备的重组β2-微球蛋白或从人尿中提取的β2-微球蛋白发生反应。根据卡巴特数据库，D1的重链可变区属于基因家族VII，轻链可变区属于κ链基因家族XI。