Eremenko V V, Mardashev S R, Tiul'panova E S, Evseev L P
Vopr Med Khim. 1976 May-Jun;22(3):334-8.
Biosynthesis of L-asparaginase (EC 3.5.1.1) was inhibited in the growing culture of Bac. mesentericus 43A on addition of L-aspartic acid (20 mM). My treatment with methyl nitrosourea (2 mg/ml) mutants were obtained, which grew poorly on aspartic acid used as the only source of carbon and nitrogen. The aspartic acid did not repress the asparaginase biosynthesis in 8 strains, found between the mutants. In six of these mutants the asparaginase biosynthesis was inhibited by means of the type of catabolite repression. The data obtained suggest that in Bac. mesentericus 43A the asparaginase biosynthesis is controlled more likely by two independent mechanisms: 1) specific repression with aspartic acid as an end product and 2) catabolite repression.
在添加L-天冬氨酸（20 mM）的情况下，肠系膜芽孢杆菌43A的生长培养物中L-天冬酰胺酶（EC 3.5.1.1）的生物合成受到抑制。用甲基亚硝基脲（2 mg/ml）处理后获得了突变体，这些突变体在以天冬氨酸作为唯一碳源和氮源的培养基上生长较差。在这些突变体中发现，天冬氨酸不会抑制8个菌株中天冬酰胺酶的生物合成。在其中6个突变体中，天冬酰胺酶的生物合成通过分解代谢物阻遏类型受到抑制。所获得的数据表明，在肠系膜芽孢杆菌43A中，天冬酰胺酶的生物合成更可能受两种独立机制控制：1）以天冬氨酸作为终产物的特异性阻遏，以及2）分解代谢物阻遏。
