Acute effect of endogenous inhibitors and exogenous cytostatics on the ultrastructure of bone marrow cells. II. Single dose of granulocyte crude extract (GCE).

The particle-free crude extract of granulocytes (GCE) contains at least three inhibitors of proliferation (M.w. greater than or equal to 70,000, approximately 11,500 and less than or equal to 4000, [5,5]). Intraperitoneal administration of a single effective mitodepressive dose (EDM = 59.4 mg/kg b.w. protein) does not damage the majority of rat bone marrow cells. The observed slight ultrastructural alterations are as follows. The cristate pattern of the mitochondria is occasionally deficient, their matrix may contain membrane shreds or myelin figures, the package of individual granules may be injured. Cytoplasmic vacuolization, autophagy are infrequent and nuclear bleb formation occurred in a single case only. The incidence of such injuries is practically the same in untreated bone marrow cells. Although GCE effectively inhibits cell proliferation, its single dose does not induce such toxic, irreversible and degenerative ultrastructural alterations as have been observed in the same system after the administration of the cytostatic dianhydrogalactitol.