Pickering E C, Williams J H, Ergene N
Res Vet Sci. 1976 Nov;21(3):259-63.
Respones to bumetanide were studied in five ewes. During ADH infusion urine flow increased from less than 1-0 to approximately 11-5 cm3 min-1 within 30 min of intravenous injection of 0-02 mg kg-1 bumetanide and returned to approximately 2-0 cm3 min-1 within 3 h of dosing. The diuresis was accompanied by large increases in sodium and chloride excretion and smaller increases in potassium and free hydrogen ion excretion. Bicarbonate excretion and TCH20 were reduced. Plasma potassium and chloride concentrations decreased slightly while arterial bicarbonate pH and pCO2 slightly increased. A transient increase in GFR and RPF was followed by a small reduction in GFR. No change in CH20 was observed after bumetanide injection during water diuresis. Increasing the dose of bumetanide over the range 0-002 to 0-20 mg kg-1 resulted in more pronounced and prolonged responses. The results show that bumetanide is a potent diuretic in sheep with its main site of action on the ascending limb of Henle's loop.
对五只母羊进行了布美他尼反应的研究。在抗利尿激素输注期间，静脉注射0.02mg/kg布美他尼后30分钟内，尿流量从小于1.0增加到约11.5cm³/min，并在给药后3小时内恢复到约2.0cm³/min。利尿伴随着钠和氯排泄的大幅增加以及钾和游离氢离子排泄的较小增加。碳酸氢盐排泄和总二氧化碳排出量减少。血浆钾和氯浓度略有下降，而动脉血碳酸氢盐pH值和pCO₂略有升高。肾小球滤过率（GFR）和肾血浆流量（RPF）短暂增加后，GFR略有降低。在水利尿期间注射布美他尼后，未观察到总二氧化碳排出量的变化。将布美他尼剂量在0.002至0.20mg/kg范围内增加会导致更明显和持久的反应。结果表明，布美他尼是绵羊体内一种强效利尿剂，其主要作用部位在亨氏袢升支。
