实验性糖尿病大鼠肝脏糖原合酶活性的变化
Changes of activity of liver glycogen synthase in experimental diabetes mellitus rats.
作者信息
Wang Y, Qian R, Ma X
机构信息
Department of Endocrinology of the First Clinical School of Beijing Medical University, China.
出版信息
Chin Med J (Engl). 1998 Jan;111(1):32-4.
OBJECTIVE
To study the activity of liver glycogen synthase in analogous model of NIDDM rats.
METHODS
Male Wistar rats were injected with low dose of streptozotocin (STZ) (30 mg/kg body weight) via tail vena and those animals with glucose tolerance impaired and level of insulin equal to or higher than that of the controls at 18th week were taken as the analogous rat model of NIDDM. The activity of liver glycogen synthase (GS) was assayed at the end of experiment.
RESULTS
Type I-enzyme: 0.18 +/- 0.06 mumol/min.g versus 0.24 +/- 0.09 mumol/min.g, P < 0.05, and total-enzyme: 1.52 +/- 0.43 mumol/min. g versus 1.84 +/- 0.42 mumol/min.g, P < 0.05.
CONCLUSION
The GS activity of diabetic rats was significantly lower than that of control.
目的
研究非胰岛素依赖型糖尿病（NIDDM）大鼠类似模型中肝糖原合酶的活性。
方法
雄性Wistar大鼠经尾静脉注射低剂量链脲佐菌素（STZ）（30毫克/千克体重），将第18周时糖耐量受损且胰岛素水平等于或高于对照组的动物作为NIDDM大鼠类似模型。在实验结束时测定肝糖原合酶（GS）的活性。
结果
I型酶：0.18±0.06微摩尔/分钟·克对0.24±0.09微摩尔/分钟·克，P<0.05；总酶：1.52±0.43微摩尔/分钟·克对1.84±0.42微摩尔/分钟·克，P<0.05。
结论
糖尿病大鼠的GS活性显著低于对照组。
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