Toma V, Sata T, Vogt P, Komminoth P, Heitz P U, Roth J
Department of Pathology, University of Zürich, Switzerland.
Cancer. 1999 May 15;85(10):2151-9.
It has been proposed that the Thomsen-Friedenreich glycotope represents a general carcinoma-associated antigen and a candidate for the development of a tumor vaccine. However, the expression of the unmasked and masked (sialylated) forms in lung carcinomas, as well as in developing and adult human lung, has not been documented sufficiently.
Sections from 82 lung carcinomas, including squamous cell carcinomas, adenocarcinomas, and large cell and small cell carcinomas, as well as sections of developing and adult human lung were studied using the lectin amaranthin and a monoclonal antibody.
All lung carcinomas but one bronchiolo-alveolar carcinoma were unreactive for the Thomsen-Friedenreich glycotope, whereas its sialylated form was detectable in well-differentiated squamous cell carcinomas and adenocarcinomas, including bronchiolo-alveolar carcinomas. Both unmasked and masked Thomsen-Friedenreich glycotopes were undetectable in large cell and small cell lung carcinomas. In all developmental stages of lung, the Thomsen-Friedenreich glycotope was expressed only in epithelia of the most peripheral parts of the bronchial tree, whereas its sialylated form was expressed in epithelia of all parts of the bronchial tree. In adult lung, the Thomsen-Friedenreich glycotope was expressed in pneumocytes, whereas its sialylated form was expressed ubiquitously in all epithelia.
The Thomsen-Friedenreich glycotope in human lung represents a differentiation antigen, rather than a carcinoma-associated antigen. The sialylated form is expressed constitutively in both developing and adult lung and well-differentiated lung carcinomas. Thus, the Thomsen-Friedenreich glycotope is of limited value in the diagnosis of lung carcinoma, and there is no rationale for a Thomsen-Friedenreich glycotope-based immunotherapy for patients with this disease.
有人提出，汤姆森 - 弗里德赖希糖表位代表一种常见的癌相关抗原，是肿瘤疫苗研发的一个候选对象。然而，肺癌以及发育中和成人的肺组织中未被掩盖和被掩盖（唾液酸化）形式的表达情况，尚未得到充分记录。
使用凝集素苋菜红和一种单克隆抗体，对82例肺癌（包括鳞状细胞癌、腺癌、大细胞癌和小细胞癌）以及发育中和成人的肺组织切片进行研究。
除一例细支气管肺泡癌外，所有肺癌对汤姆森 - 弗里德赖希糖表位均无反应，而其唾液酸化形式在高分化鳞状细胞癌和腺癌（包括细支气管肺泡癌）中可检测到。在大细胞肺癌和小细胞肺癌中，未被掩盖和被掩盖的汤姆森 - 弗里德赖希糖表位均无法检测到。在肺的所有发育阶段，汤姆森 - 弗里德赖希糖表位仅在支气管树最外周部分的上皮中表达，而其唾液酸化形式在支气管树各部分的上皮中均有表达。在成人肺中，汤姆森 - 弗里德赖希糖表位在肺细胞中表达，而其唾液酸化形式在所有上皮中普遍表达。
人肺中的汤姆森 - 弗里德赖希糖表位代表一种分化抗原，而非癌相关抗原。唾液酸化形式在发育中和成人肺以及高分化肺癌中均持续表达。因此，汤姆森 - 弗里德赖希糖表位在肺癌诊断中的价值有限，且没有理由对该疾病患者进行基于汤姆森 - 弗里德赖希糖表位的免疫治疗。
