小鼠血管活性肠肽1（VPAC1）受体的基因组结构与染色体定位

Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC1) receptor.

作者信息

Hashimoto H, Nishino A, Shintani N, Hagihara N, Copeland N G, Jenkins N A, Yamamoto K, Matsuda T, Ishihara T, Nagata S, Baba A

机构信息

Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, 565-0871, Japan.

出版信息

Genomics. 1999 May 15;58(1):90-3. doi: 10.1006/geno.1999.5805.

DOI:10.1006/geno.1999.5805

PMID:10331949

Abstract

The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC1) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kb in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'-flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped.

摘要

编码小鼠血管活性肠肽1型（VPAC1）受体的基因被克隆，并确定了其结构组织。该基因（Vipr1）长度超过16 kb，分为13个外显子。5'侧翼区域富含GC，缺乏明显的TATA盒，但包含一个CCAAT盒、三个潜在的Sp1结合位点和两个潜在的AP-2结合位点。使用荧光素酶基因报告系统对Vipr1的5'侧翼区域进行启动子分析表明，分离出的5'侧翼区域具有功能性启动子活性。小鼠Vipr1基因由单个基因编码，该基因被定位到小鼠9号染色体的远端区域。该区域与人类3号染色体p臂同线，人类VPAC1受体基因已定位在该区域。