Chakraborty R, Dasgupta D, Adhya S, Basu M K
Division of Biomembrane, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700 032, India.
Biochem J. 1999 Jun 1;340 ( Pt 2)(Pt 2):393-6.
Antisense oligonucleotides have been considered as inhibitors of growth of intracellular parasites such as Leishmania, but only limited inhibition has been observed in vitro. We have encapsulated an antisense oligonucleotide, complementary to the Leishmania universal miniexon sequence, in cationic liposomes. Low concentrations (4 microM) of encapsulated oligonucleotides specifically reduced the amastigote burden within cultured macrophages by 80%. This result illustrates the importance of effective delivery for efficient antiparasitic activity of antisense oligonucleotides.
反义寡核苷酸已被视为细胞内寄生虫(如利什曼原虫)生长的抑制剂,但在体外仅观察到有限的抑制作用。我们已将与利什曼原虫通用小外显子序列互补的反义寡核苷酸包裹于阳离子脂质体中。低浓度(4 microM)的包裹型寡核苷酸能特异性地使培养巨噬细胞内的无鞭毛体数量减少80%。这一结果说明了有效递送对于反义寡核苷酸高效抗寄生虫活性的重要性。