肠杆菌科细菌在有氧条件下对L(-)-肉碱的代谢

Metabolism of L(-)-carnitine by Enterobacteriaceae under aerobic conditions.

作者信息

Elssner T, Preusser A, Wagner U, Kleber H P

机构信息

Institut für Biochemie, Universität Leipzig, Germany.

出版信息

FEMS Microbiol Lett. 1999 May 15;174(2):295-301. doi: 10.1111/j.1574-6968.1999.tb13582.x.

DOI:10.1111/j.1574-6968.1999.tb13582.x

PMID:10339822

Abstract

Different Enterobacteriaceae, such as Escherichia coli, Proteus vulgaris and Proteus mirabilis, are able to convert L(-)-carnitine, via crotonobetaine, into gamma-butyrobetaine in the presence of carbon and nitrogen sources under aerobic conditions. Intermediates of L(-)-carnitine metabolism (crotonobetaine, gamma-butyrobetaine) could be detected by thin-layer chromatography. In parallel, L(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase activities were determined enzymatically. Monoclonal antibodies against purified CaiB and CaiA from E. coli O44K74 were used to screen cell-free extracts of different Enterobacteriaceae (E. coli ATCC 25922, P. vulgaris, P. mirabilis, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae) grown under aerobic conditions in the presence of L(-)-carnitine.

摘要

不同的肠杆菌科细菌，如大肠杆菌、普通变形杆菌和奇异变形杆菌，在有氧条件下，在有碳源和氮源存在时，能够通过巴豆甜菜碱将L(-)-肉碱转化为γ-丁酸甜菜碱。L(-)-肉碱代谢的中间产物（巴豆甜菜碱、γ-丁酸甜菜碱）可通过薄层色谱法检测。同时，通过酶法测定L(-)-肉碱脱水酶、肉碱消旋酶系统和巴豆甜菜碱还原酶的活性。使用针对大肠杆菌O44K74纯化的CaiB和CaiA的单克隆抗体，筛选在有氧条件下、L(-)-肉碱存在时培养的不同肠杆菌科细菌（大肠杆菌ATCC 25922、普通变形杆菌、奇异变形杆菌、弗氏柠檬酸杆菌、阴沟肠杆菌和肺炎克雷伯菌）的无细胞提取物。