Hogarth K, Dixon K E
J Exp Zool. 1976 Dec;198(3):429-35. doi: 10.1002/jez.1401980318.
(3H) leucine was injected into unfertilized eggs, fertilized eggs, and Stage 2-12 embryos of X. laevis. Incorporation of the leucine into protein by blastomeres containing germ plasm was studied autoradiographically. Eggs, both fertilized and unfertilized, actively synthesized protein, ad did embryos from Stage 2 onwards. Probably all blastomerers containing germ plasm were labelled. In embryos from Stages 4-12, the germ plasm itself was also labelled, and this result suggests that the germ plasm is metabolically active during cleavage.
将(3H)亮氨酸注射到非洲爪蟾的未受精卵、受精卵以及2至12期胚胎中。通过放射自显影术研究了含有生殖质的卵裂球将亮氨酸掺入蛋白质的情况。无论是受精卵还是未受精卵都能积极合成蛋白质,2期及以后的胚胎也是如此。可能所有含有生殖质的卵裂球都被标记了。在4至12期的胚胎中,生殖质本身也被标记了,这一结果表明生殖质在卵裂过程中具有代谢活性。