Turchin A, Lawler J F
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Biotechniques. 1999 Apr;26(4):672-6. doi: 10.2144/99264st02.
Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins. Newer PCR-based mutagenesis techniques have reduced the process of mutagenesis to as little as one day. While each technique has its advantages, both require a strategy to isolate the desired clone from a population that contains mutagenized and wild-type genes. In this report, we describe a World Wide Web-based computer program that facilitates the design of mutagenic primers such that successfully mutagenized clones can be identified by the presence or absence of a unique restriction site.
定点诱变是一种强大的工具，它使分子生物学家能够对改变的核酸和蛋白质进行功能分析。更新的基于聚合酶链反应（PCR）的诱变技术已将诱变过程缩短至仅一天。虽然每种技术都有其优点，但两者都需要一种策略，以便从包含诱变基因和野生型基因的群体中分离出所需的克隆。在本报告中，我们描述了一种基于万维网的计算机程序，该程序有助于设计诱变引物，以便通过独特限制酶切位点的存在或缺失来鉴定成功诱变的克隆。
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