从胚胎干细胞体外分化而来的神经元表达运动神经元和中间神经元特有的同源蛋白。

Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons.

作者信息

Renoncourt Y, Carroll P, Filippi P, Arce V, Alonso S

机构信息

INSERM U382, Developmental Biology Institute of Marseille (IBDM), CNRS/INSERM/Université de la Méditerranée/AP de Marseille, France.

出版信息

Mech Dev. 1998 Dec;79(1-2):185-97. doi: 10.1016/s0925-4773(98)00189-0.

DOI:10.1016/s0925-4773(98)00189-0

PMID:10349632

Abstract

We have characterized different neuronal subpopulations derived from in vitro differentiation of embryonic stem (ES) cells using as markers the expression of several homeodomain transcription factors. Following treatment of embryo-like aggregates with retinoic acid (RA), Pax-6, a protein expressed by ventral central nervous system (CNS) progenitors is induced. In contrast, Pax-7 expressed in vivo by dorsal CNS progenitors, and erbB3, a gene expressed by neural crest cells and its derivatives, are almost undetectable. CNS neuronal subpopulations generated expressed combinations of markers characteristic of somatic motoneurons (Islet-1/2, Lim-3, and HB-9), cranial motoneurons (Islet-1/2 and Phox2b) and interneurons (Lim-1/2 or EN1). Molecular characterization of neuron subtypes generated from ES cells should considerably facilitate the identification of new genes expressed by restricted neuronal cell lineages.

摘要

我们利用几种同源结构域转录因子的表达作为标志物，对源自胚胎干细胞（ES）体外分化的不同神经元亚群进行了特征描述。在用视黄酸（RA）处理类胚胎聚集体后，腹侧中枢神经系统（CNS）祖细胞表达的一种蛋白质——Pax-6被诱导表达。相比之下，背侧CNS祖细胞在体内表达的Pax-7以及神经嵴细胞及其衍生物表达的一个基因——erbB3几乎检测不到。所产生的CNS神经元亚群表达了躯体运动神经元（Islet-1/2、Lim-3和HB-9）、颅运动神经元（Islet-1/2和Phox2b）和中间神经元（Lim-1/2或EN1）特有的标志物组合。对ES细胞产生的神经元亚型进行分子特征描述应能极大地促进对由受限神经元细胞谱系表达的新基因的鉴定。