Pan Y, Anthony M, Clarkson T B
Comparative Medicine Clinical Research Center, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1040, USA.
Proc Soc Exp Biol Med. 1999 Jun;221(2):118-25. doi: 10.1046/j.1525-1373.1999.d01-64.x.
We report here the effects of oral micronized estradiol and soy phytoestrogens on uterine weight, choline acetyltransferase (ChAT) and nerve growth factor (NGF) mRNAs in the frontal cortex and hippocampus of ovariectomized young and retired breeder rats. Within each age category, 15 bilaterally ovariectomized rats were randomized equally into three groups: control (OVX), estradiol (E2), and soy phytoestrogens (SBE). The OVX rats were fed a casein/lactalbumin-based control diet; the E2 rats were fed with the control diet with added estradiol; and the SBE rats were fed with the control diet with added soy phytoestrogens. After 8 weeks of treatment, blood, uteri, frontal cortex, and hippocampus were collected at necropsy. Results showed that the uterine weights and serum estradiol concentrations were significantly higher in the E2 group compared with those in the OVX and SBE groups. In the hippocampus of young rats, E2 treatment resulted in significantly higher NGF mRNA levels than no treatment (OVX), and NGF mRNA levels in the SBE group were intermediate between the E2 and OVX groups. ChAT mRNA levels were significantly higher in the frontal cortex of E2 and SBE-treated retired breeder rats compared to OVX retired breeder rats. There were no differences among treatment groups for ChAT mRNA levels in the frontal cortex of young rats and in the hippocampus of both young and retired breeder rats. Our data suggest that soy phytoestrogens may function as estrogen agonists in regulating ChAT and NGF mRNAs in the brain of female rats.
我们在此报告口服微粉化雌二醇和大豆植物雌激素对去卵巢的年轻和老龄繁殖期大鼠额叶皮质和海马体中子宫重量、胆碱乙酰转移酶（ChAT）和神经生长因子（NGF）mRNA的影响。在每个年龄组中，将15只双侧去卵巢大鼠平均随机分为三组：对照组（OVX）、雌二醇组（E2）和大豆植物雌激素组（SBE）。给OVX大鼠喂食以酪蛋白/乳白蛋白为基础的对照饮食；给E2组大鼠喂食添加了雌二醇的对照饮食；给SBE组大鼠喂食添加了大豆植物雌激素的对照饮食。治疗8周后，在尸检时采集血液、子宫、额叶皮质和海马体。结果显示，与OVX组和SBE组相比，E2组的子宫重量和血清雌二醇浓度显著更高。在年轻大鼠的海马体中，E2处理导致NGF mRNA水平显著高于未处理组（OVX），SBE组的NGF mRNA水平介于E2组和OVX组之间。与OVX老龄繁殖期大鼠相比，E2和SBE处理的老龄繁殖期大鼠额叶皮质中的ChAT mRNA水平显著更高。在年轻大鼠的额叶皮质以及年轻和老龄繁殖期大鼠的海马体中，各治疗组之间的ChAT mRNA水平没有差异。我们的数据表明，大豆植物雌激素在调节雌性大鼠大脑中的ChAT和NGF mRNA方面可能起到雌激素激动剂的作用。
