胚胎期鸡软骨骨骺将抗坏血酸2 - 硫酸盐中的硫酸盐掺入硫酸软骨素的过程。

Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses.

作者信息

Hatanaka H, Egami F

出版信息

J Biochem. 1976 Dec;80(6):1215-21. doi: 10.1093/oxfordjournals.jbchem.a131392.

DOI:10.1093/oxfordjournals.jbchem.a131392

Abstract

Radioactivity was significantly incorporated from ascorbate 2-[35S]sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses. The extent of incorporation was comparable with that from inorganic [35S]sulfate. The radioactive chondroitin sulfate formed from ascorbate 2-[35S]sulfate gave two radioactive disaccharides on chondroitinase-ABC [EC 4.2.2.4] digestion. The incorporation was markedly decreased by inorganic sulfate. The time course of incorporation from ascorbate 2-[35S]sulfate and inorganic [35S]sulfate into chondroitin sulfate and the constituent disaccharides suggest that the incorporation rates from the two radioactive substances are different.

摘要

胚胎期鸡软骨骨骺能将放射性从抗坏血酸2 - [35S]硫酸盐显著掺入硫酸软骨素中。掺入程度与从无机[35S]硫酸盐的掺入程度相当。由抗坏血酸2 - [35S]硫酸盐形成的放射性硫酸软骨素经软骨素酶ABC [EC 4.2.2.4]消化后产生两种放射性二糖。无机硫酸盐能显著降低掺入量。抗坏血酸2 - [35S]硫酸盐和无机[35S]硫酸盐掺入硫酸软骨素及其组成二糖的时间进程表明，这两种放射性物质的掺入速率不同。

相似文献

1

Sulfate incorporation from ascorbate 2-sulfate into chondroitin sulfate by embryonic chick cartilage epiphyses.

J Biochem. 1976 Dec;80(6):1215-21. doi: 10.1093/oxfordjournals.jbchem.a131392.

2

Release of inorganic sulfate ion under mild alkaline conditions from sulfated, unsaturated disaccharides obtained from chondroitin sulfates by chondroitinase digestion.

Carbohydr Res. 1986 Nov 1;155:277-82. doi: 10.1016/s0008-6215(00)90159-9.

3

Simultaneous sulfation of endogenous chondroitin sulfate and chondroitin-derived oligosaccharides. Studies with separate 4-sulfating and 6-sulfating microsomal systems.

J Biol Chem. 1986 Sep 25;261(27):12659-64.

4

The effect of aging on the synthesis of hexosamine-containing substances from rat costal cartilage. A decrease in sulfation of chondroitin sulfate with aging.

J Biochem. 1979 Feb;85(2):519-28. doi: 10.1093/oxfordjournals.jbchem.a132359.

5

The sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses.

J Biol Chem. 1969 Jan 25;244(2):348-52.

6

Metabolic labeling of human thyroglobulin with [35S]sulfate: incorporation into chondroitin 6-sulfate and endoglycosidase-F-susceptible carbohydrate units.

Endocrinology. 1988 Jun;122(6):2428-35. doi: 10.1210/endo-122-6-2428.

7

Synthesis of chondroitin sulfate D and heparin proteoglycans in murine lymph node-derived mast cells. The dependence on fibroblasts.

J Biol Chem. 1990 Jul 25;265(21):12324-30.

8

Differential incorporation of sulfate into the chondroitin chain and complex carbohydrate chains of human thyroglobulin: studies in normal and neoplastic thyroid tissue.

Endocrinology. 1989 Jan;124(1):356-62. doi: 10.1210/endo-124-1-356.

9

Copolymers of chondroitin 4-sulfate and chondroitin 6-sulfate in chick embryo epiphyses and other cartilage.

J Biol Chem. 1978 Nov 10;253(21):7646-9.

10

Sequential degradation of a chondroitin sulphate trisaccharide by lysosomal enzymes from embryonic-chick epiphysial cartilage.

Biochem J. 1979 Apr 1;179(1):7-13. doi: 10.1042/bj1790007.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。