SecB伴侣蛋白对大肠杆菌周质青霉素酰化酶产生的影响。

Effect of SecB chaperone on production of periplasmic penicillin acylase in Escherichia coli.

作者信息

Chou C P, Tseng J H, Kuo B Y, Lai K M, Lin M I, Lin H K

机构信息

Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan, ROC.

出版信息

Biotechnol Prog. 1999 May-Jun;15(3):439-45. doi: 10.1021/bp990036z.

DOI:10.1021/bp990036z

PMID:10356260

Abstract

The effect of SecB chaperone on production of periplasmic penicillin acylase (PAC) in Escherichia coli was investigated. It appears that formation of PAC required the function of SecB chaperone and the amount of SecB required was at a basal level. The secB mutant was defective in production of PAC, and the impairment could be complemented by extrachromosomally supplementing SecB in trans. The function of SecB might be primarily stabilizing the cytoplasmic PAC precursors. Overproduction of SecB chaperone usually resulted in an increase in the amount of PAC precursors without enhancing PAC activity. In addition, most of the PAC precursors were located in the periplasm, suggesting that formation of active PAC was likely limited by periplasmic processing steps.

摘要

研究了SecB分子伴侣对大肠杆菌周质青霉素酰化酶（PAC）产生的影响。结果表明，PAC的形成需要SecB分子伴侣的功能，且所需的SecB量处于基础水平。secB突变体在PAC产生方面存在缺陷，通过在反式中额外补充染色体外的SecB可以弥补这种缺陷。SecB的功能可能主要是稳定细胞质中的PAC前体。SecB分子伴侣的过量表达通常会导致PAC前体数量增加，但不会提高PAC活性。此外，大多数PAC前体位于周质中，这表明活性PAC的形成可能受到周质加工步骤的限制。