非肥胖糖尿病（NOD）小鼠自身免疫性糖尿病中共享的TCR CDR3序列

A shared TCR CDR3 sequence in NOD mouse autoimmune diabetes.

作者信息

Tikochinski Y, Elias D, Steeg C, Marcus H, Kantorowitz M, Reshef T, Ablamunits V, Cohen I R, Friedmann A

机构信息

The Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

出版信息

Int Immunol. 1999 Jun;11(6):951-6. doi: 10.1093/intimm/11.6.951.

DOI:10.1093/intimm/11.6.951

PMID:10360969

Abstract

T cells involved in autoimmune diseases have been characterized by the genetic elements used to construct their autoimmune TCR. In the present study, we sequenced the alpha and beta chains of the TCR expressed by a CD4(+) T cell clone, C9, functional in NOD mouse diabetes. Clone C9 can adoptively transfer diabetes or, when attenuated, C9 can be used to vaccinate NOD mice against diabetes. Clone C9 recognizes a peptide epitope (p277) of the 60 kDa heat shock protein (hsp60) molecule. We now report that the C9 TCR beta chain features a CDR3 peptide sequence that is prevalent among NOD mice. This CDR3 element is detectable by 2 weeks of age in the thymus, and later in the spleen and in the autoimmune insulitis. Thus, a TCR CDR3beta sequence appears to be a common idiotope associated with mouse diabetes.

摘要

参与自身免疫性疾病的T细胞已通过用于构建其自身免疫性TCR的遗传元件得以表征。在本研究中，我们对一个在NOD小鼠糖尿病中发挥作用的CD4(+) T细胞克隆C9所表达的TCR的α链和β链进行了测序。克隆C9能够过继性转移糖尿病，或者当它被减毒时，C9可用于给NOD小鼠接种以预防糖尿病。克隆C9识别60 kDa热休克蛋白（hsp60）分子的一个肽表位（p277）。我们现在报告，C9 TCR β链具有在NOD小鼠中普遍存在的CDR3肽序列。这个CDR3元件在胸腺中2周龄时即可检测到，随后在脾脏和自身免疫性胰岛炎中也可检测到。因此，一个TCR CDR3β序列似乎是与小鼠糖尿病相关的一个共同独特型。