一种用于RNA功能/结构分析的化学遗传学方法。

A chemogenetic approach to RNA function/structure analysis.

作者信息

Strobel S A

机构信息

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.

出版信息

Curr Opin Struct Biol. 1999 Jun;9(3):346-52. doi: 10.1016/S0959-440X(99)80046-3.

DOI:10.1016/S0959-440X(99)80046-3

PMID:10361087

Abstract

Almost two dozen nucleotide analogs have been synthesized with alpha-phosphorothioate-tagged triphosphates and utilized in an interference modification approach termed Nucleotide Analog Interference Mapping. This method has made it possible to determine the chemical basis of RNA function and structure, including the identification of new rules for RNA helix packing, the functional analysis of a binding site for monovalent metal ions within RNA and the characterization of the catalytic mechanism of RNA enzymes.

摘要

近二十多种核苷酸类似物已通过α-硫代磷酸标记的三磷酸合成，并用于一种称为核苷酸类似物干扰图谱的干扰修饰方法。这种方法使得确定RNA功能和结构的化学基础成为可能，包括确定RNA螺旋堆积的新规则、分析RNA中单价金属离子结合位点的功能以及表征RNA酶的催化机制。