基于聚合物的基因递送系统的稳定化

Stabilization of polymer-based gene delivery systems.

作者信息

Cherng J Y, vd Wetering P, Talsma H, Crommelin D J, Hennink W E

机构信息

Research School Groningen-Utrecht Institute for Drug Exploration (GUIDE), Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80.082, 3508 TB, Utrecht, The Netherlands.

出版信息

Int J Pharm. 1999 Jun 10;183(1):25-8. doi: 10.1016/s0378-5173(99)00037-x.

DOI:10.1016/s0378-5173(99)00037-x

PMID:10361148

Abstract

To preserve the size and transfection potential of polymer-plasmid complexes. Freeze-drying and freeze-thawing were used for stabilization of these complexes. The concentration of the sugars is an important factor affecting both the size and transfection capability of the complexes after freeze-drying and freeze-thawing. However, the type of lyoprotectant (sugar) used is of minor importance. It is also shown that when damage to polymer-plasmid complexes occurs, it results from the drying process but is not due to the freezing step.

摘要

为保持聚合物-质粒复合物的大小和转染潜力，采用冻干和冻融法对这些复合物进行稳定化处理。糖的浓度是影响冻干和冻融后复合物大小及转染能力的重要因素。然而，所用冻干保护剂（糖）的类型则不太重要。研究还表明，当聚合物-质粒复合物受到损伤时，其原因在于干燥过程而非冷冻步骤。