von Marschall Z, Riecken E O, Rosewicz S
Medizinische Klinik I, Gastroenterologie/Infektiologie, Klinikum Benjamin Franklin, Berlin, Germany.
Br J Cancer. 1999 Jun;80(7):935-9. doi: 10.1038/sj.bjc.6690446.
We have investigated the effects of retinoic acid (RA) on matrix metalloprotease-1 (MMP-1) gene expression in the human pancreatic tumour cell line Dan-G. 13-cis RA results in a time- and dose-dependent increase of MMP-1 protein concentration. These stimulatory effects were paralleled by a time- and dose-dependent increase of MMP-1 mRNA steady-state concentrations. Nuclear run-on analysis revealed that the increase of MMP-1 mRNA was partially due to an increase of MMP-1 gene transcription. In addition, 13-cis RA treatment results in an increase of MMP-1 mRNA stability. These data demonstrate that RA stimulates MMP-1 gene expression in human pancreatic carcinoma cells by transcriptional and post-transcriptional mechanisms.
我们研究了视黄酸（RA）对人胰腺肿瘤细胞系Dan-G中基质金属蛋白酶-1（MMP-1）基因表达的影响。13-顺式视黄酸导致MMP-1蛋白浓度呈时间和剂量依赖性增加。这些刺激作用伴随着MMP-1 mRNA稳态浓度的时间和剂量依赖性增加。核转录分析表明，MMP-1 mRNA的增加部分归因于MMP-1基因转录的增加。此外，13-顺式视黄酸处理导致MMP-1 mRNA稳定性增加。这些数据表明，视黄酸通过转录和转录后机制刺激人胰腺癌细胞中MMP-1基因的表达。
